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This course is designed to address Historic Preservation and Conservation as an approach that establishes a link between past, present and future. To familiarize the students with the status of conservation movement, various agencies involved in the field of conservation worldwide and their policies. Introduction to International framework of conservation, definitions and concepts. Various guidelineThis course introduces to the basic biology of proteins and the new advanced science called as proteomics which aims to look into the protein properties from a global perspective, i.e., not undertaking one protein at a time, but an entire set of proteins in the milieu. The course will cover in detail the two major aspects of proteomics i.e., Gel-based proteomics and Mass spectrometry-based proteomics. The gel-based module will cover different techniques like SDS-PAGE, 2-DE, 2D-DIGE etc. These techniques had a major contribution in transition from protein chemistry to proteomics. Mass spectrometry, on the other hand, is an advanced analytical technique for accurate mass measurement. In this module, we will discuss the basics of mass spectrometry, sample preparations, liquid chromatography, hybrid mass spectrometers and quantitative proteomics techniques such as iTRAQ, SILAC and TMT using mass spectrometry. The course will also provide the basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics.s for the preservation, conservation and restoration of building, and an overview of current status of conservation and preservation in India are elaborated. Students are informed about the character and issues in our heritage towns, challenges of establishing the significance and the process of impact assessment through case studies, Concept of integrating development and heritage preservation, about opportunities and community participation are highlighted along with exposure to management and presentation of heritage buildings and sites.

Week

Topics

1.

Basics of Proteins and Proteomics

2.


Gel-based proteomics

3.


Two-dimensional gel electrophoresis (2-DE)

4.


Difference in gel electrophoresis (DIGE) & Systems Biology

5.


Basics of mass spectrometry

6.


Basics of mass spectrometry and sample preparation

7.


Quantitative proteomics

8.


Advancement in Proteomics

Any B.Sc. Or M.Sc.The target audiences of this course are required to have a basic introduction to biology.


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