Syllabus  |   Lectures  |   Downloads  |   FAQ  |   Ask a question  |  
Course Co-ordinated by IIT Roorkee
Coordinators
 
Dr. Ashwani K. Sharma
IIT Roorkee

 

Download Syllabus in PDF format



Untitled Document

Microscopy- Radioisotope techniques-Chromatographic methods-Electrophoresis-Centrifugation techniques- Spectroscopic Techniques- Polymerase Chain Reaction(PCR), DNA sequencing, ELISA.



S.No.

Topics and contents

Number of Lectures

1

Microscopy- Dark-field, Phase contrast, Fluorescence, Confocal, Polarization microscopy; Electron microscopy: TEM & SEM.

6

2

Radioisotope techniques- Basic concepts, GM and scintillation counter, autoradiography, RIA, Applications in biological science.

5

3

Chromatographic methods- General principles, Ion exchange, Gel filtration, Affinity and Gas chromatography techniques.

6

4

Electrophoresis- General principles, Horizontal & Vertical Gel electrophoresis, Isoelectric focusing, 2D, Pulse field and immuno, electrophoresis.

7

5

Centrifugation techniques- Basic principles, Different types of centrifuges, Analytical and Preparative Ultracentrifugation methods.

6

6

Spectroscopic Techniques- Electromagnetic radiations; UV-Visible, fluorescence, CD, NMR, X-ray, Atomic absorption and Flame emission spectroscopic techniques, Mass spectrometry.

7

7

Polymerase Chain Reaction, DNA sequencing, ELISA.

3

 

Total number of lectures

40

A course on Biochemistry and Biophysics.


  1. Wilson K and Walker J "Principles and Techniques of Biochemistry and Molecular Biology" 6th Ed. Cambridge University Press, 2005.

  2. Willard, H.H., Merritt L.L. Dean J.A. and Settle F.A.,"Instrumental Methods of Analysis", 7th Ed., Wadsworth Publishing Co., 1986.

  3. Van Holde, K E, Johnson, W. and Ho, P. S., "Principles of Physical Biochemistry",   Prentice Hall, 1981.

  4. Cantor, C. R. and Schimmel, W.H., "Biophysical Chemistry Part-II", Freeman & Co., 1981.

  5. Campbell, I.D. and Dwek, R. A., "Biological Spectroscopy", Benjamin Curmmings  Publication Co. Inc., 1984.

  6. Glasel, J. and Deutscher, M. B., "Introduction to Biophysical Methods for Protein and Nucleic acid Research", Academic Press, 1995.

  7. Physical chemistry by D. Friedfelder  WH Freeman and co., 1982.


Selected Research Papers.



Important: Please enable javascript in your browser and download Adobe Flash player to view this site
Site Maintained by Web Studio, IIT Madras. Contact Webmaster: nptel@iitm.ac.in